Condensation nucleation light scattering detection for biogenic amines separated by ion-exchange chromatography

A sensitive method of analysis for biogenic amines, putrescine, cadaverine, histamine and an amino acid precursor, histidine is described herein using ion-exchange chromatography and condensation nucleation light scattering d etection. The method was successfully used for the analysis of biogenic ami nes in fish samples. The method offers a number of advantages: fast elution of analytes with no need for mobile phase conductivity suppression, no der ivatization and no electrochemical activity for the analyte's detection. Th e 3 sigma detection limits for these compounds were found to range from 8 t o 20 ng/ml. (C) 1999 Published by Elsevier Science B.V. All rights reserved .