Enantioseparation of semisynthetic ergot alkaloids on vancomycin and teicoplanin stationary phases

The macrocyclic antibiotics, vancomycin and teicoplanin, were used as chira l stationary phase selectors for the enantioselective separation of semisyn thetic ergot alkaloids in reversed-phase high-performance liquid chromatogr aphy (RP-HPLC). The chromatographic behavior of the ergot preparations was investigated in order to obtain a deeper insight into the enantiodiscrimina tive process. A variety of factors, including mobile phase parameters such as the nature and concentration of the organic modifier, buffer concentrati on and pH, were examined. Conditions for the enantioseparation of real phar maceutical preparations, i.e. Lisuride, terguride and nicergoline, were fou nd. Differences in the chiral stationary phases are presented and the inter action mechanism is discussed. (C) 1999 Elsevier Science B.V. All rights re served.