Sm. Behar et al., Susceptibility of mice deficient in CD1D or TAP1 to infection with Mycobacterium tuberculosis, J EXP MED, 189(12), 1999, pp. 1973-1980
Cellular immunity against Mycobacter tuberculosis controls infection in the
majority of infected humans. Studies in mace have delineated an important
role for CD4(+) T cells and cytokines including interferon gamma and tumor
necrosis factor cr in the response to infection with mycobacteria. Recently
, the identification of CD8(+) CD1-restricted T cells that kill M. tubercul
osis organisms via granulysin and the rapid death after infection of beta 2
microglobulin deficient mice in humans has drawn attention to a critical r
ole for CD8+ T cells. The nature of mycobacterial-specific CD8(+) T cells h
as been an enigma because few have been identified in any species. Here, we
delineate the contribution of class I MHC-restricted T cells in the defens
e against tuberculosis as transporter associated with antigen processing (T
AP)1-deficient mice died rapidly, bore a greater bacterial burden, and had
more severe tissue pathology than control mice. In contrast, CD1D(-/-) mice
were not significantly different in their susceptibility to infection than
control mice. This data demonstrates a critical role for TAP-dependent pep
tide antigen presentation and provides further evidence that class I MHC-re
stricted CD8+ T cells, the major T cell subset activated by this antigen pr
ocessing pathway, play an essential role in immunity to tuberculosis.