High-performance liquid chromatographic determination of amfepramone hydrochloride, mazindol, and diazepam in tablets

Simple and rapid procedures were developed for the quantification of amfepr amone hydrochloride and diazepam and mazindol and diazepam in tablets using high performance liquid chromatography (HPLC) with UV detection. These tec hniques provided conditions for the separation of each active ingredient fr om the complex matrices of the dosage forms by dilution or extraction in me thanol. Isocratic reversed phase chromatography was performed using acetoni trile, methanol, and aqueous 0,1% ammonium carbonate (70:10:20, v/v/v) as a mobile phase, Radial-Pak C-18 column (100 x 8 mm id, 4 mu m), a column tem perature of 25+/-1 degrees C and detection at 255 nm. The calibration curve s were linear over a wide concentration range (100-1000 mu g.mL(-1) to amfe pramone hydrochloride and mazindol and 10-100 mu g.mL(-1) to diazepam) with good correlation factors of 0.9978, 0.9956 and 0.9997 for amfepramone hydr ochloride, mazindol, and diazepam, respectively. Mean recoveries obtained from the two kinds of samples ranged from 83.2 to 102.5%, with coefficients of variation ranging from 1.0 to 6.1. These results demonstrated the efficiency of the proposed methods, as well as advantages such as simplicity and short duration of analysis.