We present a cost-effective, environmentally safer tissue processing t
echnique that results in superior morphological detail and preserves a
ntigenicity. Our procedure uses par affin oil in place of common clear
ing reagents such as xylene or limonene. Paraffin oil is compatible wi
th existing laboratory equipment and can be used in closed tissue proc
essing instruments. The oil is economical because it is easily and qui
ckly restored for repeat use.