Optimization of cultural conditions for production of lignin peroxidases by Phanerochaete chrysosporium

Phanerochaete chrysosporium, a white-rot basidiomycete, produces the lignin olytic system in idiophase upon starvation for carbon, nitrogen or sulphur. The ligninolytic system consists of two major classes of enzymes, viz lign in peroxidases (LIP) and manganese-dependent peroxidases (MnP). In the pres ent work, the synthesis and secretion of LIP were studied in different cond itions of agitation, aeration and immobilization. The influence of temperat ure shift and inducers in addition to LIP production were also evaluated. T he results showed that the mycelium immobilized on porous ceramic supports (Raschig rings or Berl saddles) under non-agitated culture, with pure oxyge n flushed daily, on nitrogen-limited medium and a temperature shift during the course of fermentation, provided optimal;conditions for LiP production.