Nucleotide sequence analysis of the 3 '-terminal region of two Korean isolates of lily symptomless carlavirus and expression of the coat protein in E-coli

The 3'-terminal regions of the genomic RNAs of two Korean isolates of the l ily symptomless Carlavirus (LSV), LSV-Ko and LSV-KII, were cloned and their nucleotide sequences were determined. The nucleotide sequence analysis and protein analysis by the Western blot revealed that E, coli expressed a 32- kDa protein that is the viral coat protein (CP) for the LSV, The two Korean strains share 98.4% and 98.3% sequence identities at the nucleotide and am ino acid levels, respectively. The CP gene of LSV-Ko showed 99.1% and 87.0% nucleotide sequence identities, and 99.0% and 96.6% amino acid sequence id entities with those of the Netherlands and the Japanese LSV strains, respec tively. A pairwise amino acid sequence comparison revealed a sequence simil arity of 29.6% to 69.8% between LSV-Ko and other species of the carlavirus. The 16 kDa protein of LSV-Ko shares 17.6% to 42.7% amino acid similarity w ith those of 8 other the carlaviruses, and they are variable in the N-termi nal region. The Cys repeated zinc finger nucleic acid binding domain was fo und in the 16 kDa protein for all of the LSV strains. Sequence comparisons of the 7 kDa protein of LSV in the strain level showed significant identiti es from 100.0% to 98.4%. LSV-Ko shares 21.9% to 42.2% amino acid similarity with those of 8 other carlaviruses, 4 members of the potexviruses, and a c losterovirus. LSV is closely related to blueberry scorch virus (BISV) based upon the phylogenetic tree analyses of the three proteins, indicating LSV to be a quite distinct member of the genus Carlavirus.