Regeneration of plants from seed derived callus of Hybanthus enneaspermus L-Muell., a rare ethnobotanical herb

Citation
E. Prakash et al., Regeneration of plants from seed derived callus of Hybanthus enneaspermus L-Muell., a rare ethnobotanical herb, PL CELL REP, 18(10), 1999, pp. 873-878
Citations number
21
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
07217714 → ACNP
Volume
18
Issue
10
Year of publication
1999
Pages
873 - 878
Database
ISI
SICI code
0721-7714(199906)18:10<873:ROPFSD>2.0.ZU;2-U
Abstract
A procedure was developed for plant regeneration of Hybanthus enneaspermus, a rare ethnobotanical herb from the Deccan peninsula in India, through see d-derived callus. Seeds demonstrated a high induction frequency (69.4+2.8%) and a high yield (364.4+/-2.5 mg) of light-yellow friable callus on Murash ige and Skoog's (MS) medium containing 2.6 mu M NAA and 2.2 mu M BA within 4 weeks of incubation. After I year of subculture, yellow friable and light -green compact calli types were established from initial light-yellow friab le callus. Shoot differentiation was achieved from light-green compact call us, but not from yellow friable callus. Shoot differentiation resulted when light-green compact callus was transferred to MS medium supplemented with 8.8 mu M BA and 2.6 mu M NAA; the highest percentage of calli forming shoot s (66.6+/-4.8%) and the highest number of shoots (8.9+/-0.3) were achieved in this medium. Differentiated shoot buds elongated to 4-5 cm within 4 week s. The addition of casein hydrolysate (500 mg/l) and more potassium phospha te (1.86 mM) to the culture medium enhanced shoot differentiation. Rooting was achieved on the shoots using half-strength MS medium containing 4.8 mu M IBA. About 70% of the plants were established in pots containing pure gar den soil after 2 weeks of hardening. The regenerated plants were morphologi cally uniform and exhibited normal seed set.