B. Roux et R. Mackinnon, The cavity and pore helices the KcsA K+ channel: Electrostatic stabilization of monovalent cations, SCIENCE, 285(5424), 1999, pp. 100-102
The electrostatic influence of the central cavity and pore alpha helices in
the potassium ion channel from Streptomyces lividans (KcsA K+ channel) was
analyzed by solving the finite difference Poisson equation. The cavity and
helices overcome the destabilizing influence of the membrane and stabilize
a cation at the membrane center. The electrostatic effect of the pore heli
ces is large compared to that described for water-soluble proteins because
of the low dielectric membrane environment. The combined contributions of t
he ion self-energy and the helix electrostatic field give rise to selectivi
ty for monovalent cations in the water-filled cavity. Thus, the K+ channel
uses simple electrostatic principles to solve the fundamental problem of io
n destabilization by the cell membrane lipid bilayer.