ENANTIOSEPARATION OF REDUCED HALOPERIDOL BY CAPILLARY ZONE ELECTROPHORESIS WITH DIMETHYL-BETA-CYCLODEXTRIN

Chiral separation of reduced haloperidol (RHP), an optically active me tabolite from haloperidol, by capillary electrophoresis is described. RHP was separated on an uncoated capillary with dimethyl-beta-cyclodex trin (dimethyl-beta-CD) in Tris phosphate buffer using PEG 6000 as an organic additive. Several parameters affecting the separation of RHP w ere studied, including pH and concentration of buffer, buffer additive , and concentration of chiral selector. The migration order of the res olved RHP was assigned with reference RHP enantiomers.