Functionalized polypyrroles: a sophisticated glue for the immobilization and electrical wiring of enzymes.

The concept and potentialities of two original electrochemical procedures o f enzyme immobilization based on functionalized polypyrroles are described. A two-step method consists of the adsorption of aqueous amphiphilic pyrrol e monomerenzyme mixtures on an electrode surface followed by electropolymer ization of the adsorbed amphiphilic monomers. Example of multienzyme entrap ment and copolymer elaboration as well as electrical wiring of immobilized enzymes are presented. The second method is based on the electropolymerizat ion of a pyrrole-modified biotin. The resulting polymer was successfully us ed for successive attachment of avidin and biotinylated enzymes.