IGE RESPONSE TO FUR ANIMAL ALLERGENS AND DOMESTIC-ANIMAL ALLERGENS INFUR FARMERS AND FUR GARMENT WORKERS

Objective The aim of this study was to compare the IgE response to the most commonly farmed fur animals with that to domestic animals. Metho ds IgE-immunoblotting and RAST-inhibition analyses were performed usin g RAST-positive sera from fur workers sensitized to fur allergens and sera from patients sensitized to domestic animal allergens. Results Th e urine extracts of mink, blue fox, silver fox, racoon dog and fitchew contained more protein bands than the fur extracts did. Allergens wit h the same molecular weight were found in ail of the fur and urine ext racts. The most prominent allergenic bands had molecular weights of 62 -67 kDa, 23-25 kDa and 18-19 kDa. With crossreacting sera the reciproc al RAST inhibition with all five animal extracts indicated common IgE- binding epitopes, probably common allergens (especially the 62-67 kDa bands). Urine and fur contain common allergens, since urine allergens strongly inhibited the IgE-binding to fur allergens. The IgE binding t o allergenic bands of fur animal extracts was also observed in immmuno blotting when dog and cat RAST-positive sera were used, but not for co w RAST-positive sera. RAST inhibition of dog-positive sera with fur an imal extracts and fur-positive sera with dog extract confirmed the cro ssreactivity of these IgE antibodies. No such inhibition was seen with cow extract. Conclusion The results of the RAST inhibition and immuno blotting suggest that fur animals have IgE binding epitopes or allerge ns in common with cat and dog - possibly albumin - but not with cow.