Potential of DNA markers in detecting divergence and in analysing heterosis in Indian elite chickpea cultivars

Molecular markers such as RAPDs and microsatellites were used to study gene tic diversity in 29 elite Indian chickpea genotypes. In general, microsatel lites were more efficient than the RAPD markers in detecting polymorphism i n these genotypes. Among the various microsatellites, (AAC)(5), (ACT)(5), ( AAG)(5) and (GATA)(4) were able to differentiate all 29 chickpea cultivars. The mean value of probability of identical match by chance was 2.32 x 10(- 25) using DraI-(ACT)(5), TaqI-(AAC)(5), TaqI-(AAG)(5) and TaqI-(GATA)(4) en zyme-probe combinations. The dendrogram, constructed on the basis of simila rity index values, grouped the chickpea genotypes into five main clusters w ith 8 cultivars genetically distant and outgrouped from the main clusters. To investigate if DNA markers are useful in predicting F-1 performance and heterosis in chickpea, we crossed 8 genotypes having important agronomic ch aracters in a diallel set. The F(1)s and their parents in the diallel set w ere analysed for agronomic traits for better parent and midparent heterosis . Heterosis was found to be much higher for yield than for yield components that fit a multiplicative model. The analysis of genetic divergence using D-2 statistics clustered the 8 cultivars into two groups. Although molecula r marker-based genetic distance did not linearly correlate to heterosis, tw o heterotic groups could be identified on the basis of the general marker h eterozygosity.