Plasma GH, IGF-I, and conception rate in cattle treated with low doses of recombinant bovine GH

Blood and uterine concentrations of GH and insulin-like growth factor (IGF) -I are correlated with improved fertility in cattle. We tested incremental doses of a 14-d sustained release recombinant bovine GH (rbGH) to increase blood CH and ICF-I (Experiments 1 and 2). Conception rate after administrat ion of an optimized rbGH dose was also tested (Experiment 3). In Experiment 1, lactating Holstein cows (n = 18) were randomly assigned to receive 0 (n = 5), 100 (n = 5), 200 (n = 5), or 500 (n = 3) mg sc rbGH. Increasing the doses of rbGH was associated with increased serum concentrations of GH and ICF-I. The 100- and 200-mg doses caused an ICE-I release that was below and above, respectively, the perceived optimum response. Therefore, Experiment 2 was designed to test a rbGH dose (167 mg), which was intermediate to the doses tested in Experiment 1. Lactating and nonlactating postpartum beef c ows were treated with 0 (n = 9) or 167 (n = 9) mg rbGH at insemination. Pla sma concentrations of CH and ICE-I were greater in rbGH-treated cows than i n controls. Lactating cows had initial ICE-I concentrations that were lower than nonlactating cows. The 167-mg dose of rbGH increased plasma ICE-I con centrations in lactating cows to the levels of those of nonlactating cows. In Experiment 3, cows and heifers were administered either 0 or 167 mg rbGH at insemination. The conception rate for rbGH-treated and control cows was 54.4 and 49.5% (n = 617), and 46.0 and 46.3% for heifers (n = 1123), respe ctively. Herd (P < 0.01) and parity (P < 0.01) affected conception rate, bu t conception rates for rbGH and control cattle were similar. In summary, lo w doses of rbGH increased blood GH and restored blood ICE-I concentrations in lactating cows to those of nonlactating cows, but the conception rate in cows and heifers was not affected by administration of 14-d sustained-rele ase rbGH at insemination. (C) 1999 by Elsevier Science Inc.