Influence of antigenic forms and adjuvants on the IgG subclass antibody response to Aujeszky's disease virus in mice

The influence of antigenic forms of Aujeszky's disease virus (ADV) and adju vant types on the production of IgG subclass antibodies in mice was investi gated. Particulate antigen, inactivated ADV, alone induced IgG1 and lower I gG2a antibody production, while the antigen adsorbed onto aluminum phosphat e gel (alum) enhanced IgG1 antibody production but suppressed IgG2a antibod y production as well as solubilized ADV antigen adsorbed onto alum. QS21 sa ponin purified from Quillaja saponaria promoted the production of IgG1 and IgG2a antibodies in a large extent against the both particulate and soluble antigens, while this saponin has strong hemolytic activity. Lablaboside F saponin isolated from Dolichos lablab without hemolytic activity. also indu ced the production of large IgG1 and little IgG2a antibody against both ant igens. Oil-based adjuvant, ISA70 of water-in-oil type and ISA25 of oil-in-w ater type, increased IgG1 and IgG2a antibodies against the both soluble and particulate antigens, whereas a combination of ISA25 and soluble antigen r educed IgG2a antibody response. These results indicate that IgG1 antibody p roduction was not suppressed by a combination of antigenic form and adjuvan t type, however, IgG2a antibody production was influenced, (C) 1999 Publish ed by Elsevier Science Ltd. All rights reserved.