Cloning of a centromere binding factor 3d (CBF3D) gene from Candida glabrata

The gene encoding centromere binding factor 3d (CBF3D) of the human pathoge nic yeast Candida glabrata has been isolated by hybridization of Saccharomy ces cerevisiae CBF3D (ScCBF3D) DNA to a C. glabrata partial genomic library . Sequence analysis revealed a 540 bp open reading frame encoding a protein of 179 amino acids with a calculated molecular mass of 20.9 kDa. The amino acid sequence is highly homologous (78.6% identity) to ScCbf3d and 48.3% i dentical to the human homologue p19 (SKP1). Southern blot analysis indicate s that CgCbf3d is encoded by an unique nuclear gene. The cloned CgCBF3D gen e can functionally substitute the S. cerevisiae homologue in a S. cerevisia e CBF3D-deletion mutant. The GenBank Accession No. for this gene is AF 0724 72. Copyright (C) 1999 John Wiley & Sons, Ltd.