Human urine samples were fractionated to examine the contribution of v
olatiles to the individual body odor. The samples were obtained from 4
male donors and fractionated using a vacuum technique. The volatiles
from the chemical fractions were analyzed using the CLSA technique and
the gas chromatography. Thereafter, these fractions were tested in a
computer-controlled olfactometer by trained rats. Although the rats we
re able to discriminate the distillation residue, they could not recog
nize the urine odor in the distilled fraction. The results of gas chro
matography indicate a continuous release of volatile constituents in t
he distillation residue. (C) 1997 Elsevier Science Inc.