Cu(III)-polypeptide complexes exhibiting SOD-like activity

Citation
M. Liakopoulou-kyriakides et al., Cu(III)-polypeptide complexes exhibiting SOD-like activity, AMINO ACIDS, 16(3-4), 1999, pp. 415-423
Citations number
10
Categorie Soggetti
Biochemistry & Biophysics
Journal title
AMINO ACIDS
ISSN journal
09394451 → ACNP
Volume
16
Issue
3-4
Year of publication
1999
Pages
415 - 423
Database
ISI
SICI code
0939-4451(1999)16:3-4<415:CCESA>2.0.ZU;2-6
Abstract
The SOD-like activity of Cu(III)-complexes with polypeptides poly-L-lysine and poly-L-glutamic acid respectively was investigated. The Cu(II)-polypept ide complexes were first oxidized by K2IrCl6 to give the corresponding Cu(I II)-compounds. The oxidation of Cu(II) and the corresponding Cu(II)/Cu(III) potential was evaluated by cyclic voltammetry (c.v.), UV-Vis and EPR spectroscopic (r.t.) experiments. Spin trapping EPR spectra were also conducted to confirm the formation of the superoxide radical. The SOD-like activity of each Cu(III)- complex was proved using the nitro blue tetrazolium (NBT) method slightly m odified.