Jp. Bossavy et al., Nonfractionated heparin fails to inhibit arterial thrombosis in a human exvivo thrombosis model, ANN VASC S, 13(4), 1999, pp. 393-401
The effect of nonfractionated heparin on the formation and composition of a
rterial thrombus is unclear. The purpose of this study in a human ex vivo m
odel was to analyze fibrinoplatelet thrombi and test the inhibitory effect
of nonfractionated heparin on arterial thrombus formation. Experiments were
carried out in Sakariassen perfusion chambers. Strips coated with either t
issue factor (TF) or collagen were exposed to human blood collected from he
althy volunteers at an arterial shear stress rate of 2600 s(-1) for 1 to 4
min. Platelet deposition was determined using immunoenzymatic techniques to
quantify P-selectine, a platelet membrane receptor, in thrombi. Fibrin dep
osition was determined by quantifying fibrin degradation products released
after application of plasmin (D-dimers). Heparin was injected into the bloo
d flow through a blender port system located between the venous puncture si
te and perfusion chamber. The results of the study showed that in a human e
x vivo model, formation of arterial thrombus on two thrombogenic surfaces (
tissue factor and collagen) is not inhibited by nonfractionated heparin.