Identification of a novel beta-lactamase produced by Xanthomonas campestris, a phytopathogenic bacterium

Authors
Weng, SF Chen, CY Lee, YS Lin, JW Tseng, YH
Citation
Sf. Weng et al., Identification of a novel beta-lactamase produced by Xanthomonas campestris, a phytopathogenic bacterium, ANTIM AG CH, 43(7), 1999, pp. 1792-1797
Citations number
27
Categorie Soggetti
Microbiology
Journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN journal
00664804 → ACNP
Volume
43
Issue
7
Year of publication
1999
Pages
1792 - 1797
Database
ISI
SICI code
0066-4804(199907)43:7<1792:IOANBP>2.0.ZU;2-N
Abstract
The Xanthomonas campestris pv. campestris 11 chromosome encodes a periplasm ic beta-lactamase of 30 kDa. Gene replacement and complementation confirmed the presence of this enzyme. Its deduced amino acid sequence shows identit y and conserved domains between it and Stenotrophomonas maltophilia L2 and other Ambler class A/Bush group 2 beta-lactamases. Southern hybridization d etected a single homologous fragment in each of 12 other Xanthomonas strain s, indicating that the presence of a beta-lactamase gene is common among xa nthomonads.