Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diazl-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl] pyrrolidine-2- carboxylic acid (LY29) and diphenyliodonium chloride (DIC) on the degradati on of protein to ammonia were determined in a mixed rumen microbial populat ion taken from sheep on a grass hay-concentrate diet. Both compounds decrea sed NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high activity ammonia-producing species, Clost ridium aminophilum and Clostridium sticklandii.