Genetic basis of enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprint pattern in Sinorhizobium meliloti and identification of S-meliloti employing PCR primers derived from an ERIC-PCR fragment
S. Niemann et al., Genetic basis of enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprint pattern in Sinorhizobium meliloti and identification of S-meliloti employing PCR primers derived from an ERIC-PCR fragment, ARCH MICROB, 172(1), 1999, pp. 22-30
The enterobacterial repetitive intergenic consensus (ERIC)-PCR method was e
mployed to generate genomic amplification products of Sinorhizobium melilot
i strain 2011. Eleven distinctive PCR fragments obtained in PCR reactions b
y using the ERIC2 primer were cloned and their partial or complete nucleoti
de sequences established. DNA sequences that extended past the ERIC2 primer
region were not conserved among the 11 PCR fragments and showed no sequenc
e similarity to the enterobacterial ERIC consensus sequence. Thus, repetiti
ve ERIC or ERIC-like sequences seem not to be an integral part of the S. me
liloti genome. An amplification product of S.,meliloti 2011 was identified
which was present in S. meliloti strains but absent in other I rhizobial sp
ecies. Based on the nucleotide sequence information, a pair of PCR primers
was designed and used for PCR amplification of sequences of S, meliloti lab
oratory strains 2011, L5-30, AK631 and 102F34. Nucleotide sequence analysis
of the amplification products revealed a 100% DNA sequence conservation. D
atabase searches showed that the DNA fragment putatively encodes the C-term
inal part of a protein displaying similarity to 2-hydroxyacid dehydrogenase
s of various organisms. The newly designed PCR primers should be useful for
the rapid identification of S. meliloti isolates.