DNA-induced conformational change of Gaf1, a novel GATA factor in Schizosaccharomyces pombe

Authors
Won, M Hoe, KL Cho, YS Song, KB Yoo, HS
Citation
M. Won et al., DNA-induced conformational change of Gaf1, a novel GATA factor in Schizosaccharomyces pombe, BIOC CELL B, 77(2), 1999, pp. 127-132
Citations number
38
Categorie Soggetti
Cell & Developmental Biology
Journal title
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
ISSN journal
08298211 → ACNP
Volume
77
Issue
2
Year of publication
1999
Pages
127 - 132
Database
ISI
SICI code
0829-8211(1999)77:2<127:DCCOGA>2.0.ZU;2-S
Abstract
A novel GATA factor in Schizosaccharomyces pombe, Gaf1, containing one zinc -finger motif was studied for conformational change that was induced by DNA -binding. Gaf1 was shown to bind to the upstream activation sequence of a g ene in Saccharomyces cerevisiae containing GATA element by gel mobility shi ft assay. Circular dichroism spectra of Gaf1 indicated an increase of a-hel ix content of Gaf1 occurred upon binding to the upstream activation sequenc e. These results suggest that the binding of Gaf1 to the GATA element is re quired for the conformational change that may precede transactivation of th e target gene(s).