Rapid continuous colorimetric enzyme assay for penicillin G acylase

Authors
Simons, H Gibson, TD
Citation
H. Simons et Td. Gibson, Rapid continuous colorimetric enzyme assay for penicillin G acylase, BIOTECH TEC, 13(6), 1999, pp. 365-367
Citations number
5
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOTECHNOLOGY TECHNIQUES
ISSN journal
0951208X → ACNP
Volume
13
Issue
6
Year of publication
1999
Pages
365 - 367
Database
ISI
SICI code
0951-208X(199906)13:6<365:RCCEAF>2.0.ZU;2-8
Abstract
A rapid, continuous, colorimetric enzyme assay for penicillin G acylase has been developed. The assay measures the formation of the acidic products of penicillin G hydrolysis by following the decrease in pH using Phenol Red a s an indicator. The activity measured is directly proportional to the amoun t of enzyme added to the assay, having a linear relationship with an R-2 va lue of 0.9994.