Lipase immobilisation on to polymeric membranes

Lipase (EC 3.1.1.3) from Candida rugosa was covalently immobilised on to ce llulose, cellulose derivatives (cellulose acetate and cellulose phthalate) and cellulose composite membranes using activating agents such as sodium pe riodate or carbodiimide. Other non-cellulosic polymeric membranes (nylon, p olyurethane, chitosan and hydroxyethyl methacrylate-co-methyl methacrylate) were also prepared and used for lipase immobilisation. The results obtaine d showed that the expressed activities are of the same order of magnitude f or similar enzyme loadings when compared with those obtained from literatur e.