Production of wheat haploids using anther culture and wheat x maize hybridization techniques

Citation
Rs. Sadasivaiah et al., Production of wheat haploids using anther culture and wheat x maize hybridization techniques, CEREAL RES, 27(1-2), 1999, pp. 33-40
Citations number
29
Categorie Soggetti
Agriculture/Agronomy
Journal title
CEREAL RESEARCH COMMUNICATIONS
ISSN journal
01333720 → ACNP
Volume
27
Issue
1-2
Year of publication
1999
Pages
33 - 40
Database
ISI
SICI code
0133-3720(1999)27:1-2<33:POWHUA>2.0.ZU;2-Q
Abstract
Ten spring wheat breeding lines derived from diverse crosses differed signi ficantly in their response to anther culture and wheat x maize hybridizatio n techniques for the production of haploids. Depending on the genotype, the frequency of embryo induction from cultured anthers ranged from 0.1 to 55. 6 per 100 anthers with a mean of 16.3. Plant regeneration from cultured emb ryos also varied with the genotype. Although the embryos from one line fail ed to regenerate, those from the nine other lines produced both green and a lbino plantlets. The frequency of green plants produced per 100 cultured an thers ranged from 0 to 4.73 with a mean of 1.64. Depending on the genotype, 25 to 75% of the anther-derived green plants showed spontaneous chromosome doubling. The wheat x maize hybridization technique was highly effective i n producing haploids in all ten breeding lines, although the frequency vari ed with genotype. The percentage of seed set ranged from 52.7 to 71.6 with a mean of 62.5, but only 19.3% of the seeds contained embryos. About 75% of the cultured embryos developed into green plants. The number of green plan ts produced per 100 pollinated florets ranged from 2.40 to 12.66 with a mea n of 6.29, all having the haploid chromosome number of 21. The line recalci trant to anther culture produced the highest number of haploids with the wh eat x maize hybridization method. There was no evidence of albinism or spon taneous chromosome doubling. The reduced genotype specificity, absence of a lbinism and ease of application, makes the wheat x maize hybridization tech nique more efficient than anther culture for the production of haploids in common wheat.