The green fluorescent protein (GFP) gene from the jellyfish Aequorea victor
ia as a vital reporter for gene expression in plants is considered to have
several advantages over other reporter genes. The pBIN35S-mGFP4 plasmid DNA
has been introduced into cotton embryos by the pollen-tube pathway method.
A transformed seedling has been verified according to its GFP-related fluo
rescence and Southern blotting analysis. The results provided direct and co
nvincing facts in cytology and molecular biology for the pollen-tube pathwa
y method, an efficient transformation technique used in plants.