Background: The conventional method of measuring total body water by the de
uterium isotope dilution method uses gas isotope ratio mass spectrometry (I
RMS), which is both expensive and time-consuming. We investigated an altern
ative method, using Fourier transform infrared spectrophotometry (FTIR), wh
ich uses less expensive instrumentation and requires little sample preparat
ion.
Method: Total body water measurements in human subjects were made by obtain
ing plasma, saliva, and urine samples before and after oral dosing with 1.5
mol of deuterium oxide. The enrichments of the body fluids were determined
from the FTIR spectra in the range 1800-2800 cm(-1), using a novel algorit
hm for estimation of instrumental response, and by IRMS for comparison.
Results: The CV (n = 5) for repeat determinations of deuterium oxide in bio
logical fluids and calibrator solutions (400-1000 mu mol/mol) was found to
be in the range 0.1-0.9%. The use of the novel algorithm instead of the int
egration routines supplied with the instrument gave at least a threefold in
crease in precision, and there was no significant difference between the re
sults obtained with FTIR and those obtained with IRMS.
Conclusion: This improved infrared method for measuring deuterium enrichmen
t in plasma and saliva requires no sample preparation, is rapid, and has po
tential value to the clinician. (C) 1999 American Association for Clinical
Chemistry.