Ss. Kakar et L. Jennes, Molecular cloning and characterization of the tumor transforming gene (TUTR1): a novel gene in human tumorigenesis, CYTOG C GEN, 84(3-4), 1999, pp. 211-216
We cloned and sequenced the cDNA of a potent tumor transforming gene (TUTR1
) from human testis and determined its primary structure. The TUTR1 cDNA is
composed of 656 nucleotides and encodes a novel protein of 202 amino acids
. The predicted TUTR1 protein is extremely hydrophilic and contains two pro
line-rich motifs at its C-terminus. Northern blot analysis of the mRNA from
various human tissues and tumors revealed that TUTR1 mRNA is highly expres
sed in tumors of the pituitary gland, adrenal gland, ovary, endometrium, li
ver, uterus, and kidney as well as in cell lines derived from tumors of the
pituitary, breast, endometrium, and ovary. With the exception of the testi
s, the levels of TUTR1 mRNA were either very low or undetectable in normal
human tissues. Overexpression of TUTR1 in mouse fibroblasts (NIH 3T3) cells
resulted in an increase in cell proliferation, induced cellular transforma
tion in vitro, and promoted tumor formation in nude mice. These results sug
gest that TUTR1 is a novel and potent transforming gene, which may be invol
ved in tumorigenesis in numerous different human tumors.