Regulation of left-right asymmetries in the zebrafish by Shh and BMP4

Left-right (LR) asymmetry of the heart in vertebrates is regulated by early asymmetric signals in the embryo, including the secreted signal Sonic hedg ehog (Shh), but less is known about LR asymmetries of visceral organs. Here we show that Shh also specifies asymmetries in visceral precursors in the zebrafish and that cardiac and visceral sidedness are independent. The tran scription factors fli-1 and Nkx-2.5 are expressed asymmetrically in the pre cardiac mesoderm and subsequently in the heart; an Eph receptor, rtk2, and an adhesion protein, DM-GRASP, mark early asymmetries in visceral endoderm. Misexpression of shh mRNA, or a dominant negative form of protein kinase A , on the right side reverses the expression of these asymmetries in precurs ors of both the heart and the viscera. Reversals in the heart and gut are u ncoordinated, suggesting that each organ interprets the signal independentl y. Misexpression of Bone Morphogenetic Protein (BMP4) on the right side rev erses the heart, but visceral organs are unaffected, consistent with a func tion for BMPs locally in the heart held. Zebrafish mutants with midline def ects show independent reversals of cardiac and visceral laterality. Thus, h h signals influence the development of multiple organ asymmetries in zebraf ish and different organs appear to respond to a central cascade of midline signaling independently, which in the heart involves BMP4. (C) 1999 Academi c Press.