The use of microsatellite polymorphisms for the identification of Australian breeding lines of rice (Oryza sativa L.)

Ten microsatellite loci were analysed for 43 cultivars or breeding lines of rice. Polymorphism-Information-Content values ranged from 0.62 to 0.92. Th e microsatellite markers were found to be useful for cultivar identificatio n and assessment of genetic relationships. Most of the cultivars could be u niquely identified by at least one microsatellite marker. Genetic heterogen eity was detected within rice samples by amplification of microsatellites f rom DNA extracted from multiple individual plants and also from bulked DNA preparations.