J. Stolz et al., Structural analysis of a plant sucrose carrier using monoclonal antibodiesand bacteriophage lambda surface display, FEBS LETTER, 453(3), 1999, pp. 375-379
Monoclonal antibodies mere raised and selected against recombinant Plantago
major PmSUC2 sucrose carrier protein, Epitopes of two monoclonal antibodie
s (PS2-1A2 and PS2-4D4) were mapped using N-terminally truncated PmSUC2 pro
teins and a lambda library displaying random PmSUC2 peptides, PS2-1A2 recog
nizes an octapeptide close to the N-terminus of PmSUC2, PS2-4D4 binds to a
decapeptide at the very C-terminus, Analyses of antibody binding to yeast p
rotoplasts with functionally active, tagged PmSUC2 protein revealed that bo
th epitopes are located in cytoplasmic domains of PmSUC2, These results sup
port a model for plant sucrose transporters containing 12 transmembrane hel
ices with the N-terminus and the C-terminus on the cytoplasmic side of the
plasma membrane. (C) 1999 Federation of European Biochemical Societies.