M. Radermacher et al., Molecular architecture of Manduca sexta midgut V-1 ATPase visualized by electron microscopy, FEBS LETTER, 453(3), 1999, pp. 383-386
The structure of the V-1 ATPase from the tobacco hornworm Manduca sexta has
been determined from electron micrographs of isolated, negatively stained
specimens. The resulting images clearly show a pseudohexagonal arrangement
of six equal-sized protein densities, presumably representing the three cop
ies each of subunits A and B, which comprise the headpiece of the enzyme. A
seventh density could be observed either centrally or asymmetrically to th
e hexamer, The maximum diameter of the V-1 complex in the hexagonal project
ion is 13 nm with each of the six peripheral densities being 3-4 nm in diam
eter. (C) 1999 Federation of European Biochemical Societies.