Citation
A. Bottin et al., Green fluorescent protein (GFP) as gene expression reporter and vital marker for studying development and microbe-plant interaction in the tobacco pathogen Phytophthora parasitica var. nicotianae, FEMS MICROB, 176(1), 1999, pp. 51-56
Citations number
16
Categorie Soggetti
Microbiology
Journal title
FEMS MICROBIOLOGY LETTERS
ISSN journal
03781097
→ ACNP
Volume
176
Issue
1
Year of publication
1999
Pages
51 - 56
Database
ISI
SICI code
0378-1097(19990701)176:1<51:GFP(AG>2.0.ZU;2-4
Abstract
PEG-mediated transformation of protoplasts in the presence of lipofectin wa
s achieved in Phytophthora parasitica var. nicotianae, an oomycete pathogen
of tobacco. Using oomycete promoter and terminator sequences, a plant-adap
ted green fluorescent protein (GFP) was introduced into the microorganism.
The data show for the first time that this eukaryotic gene reporter can be
used in an oomycete, both as a quantitative reporter of gene induction and
as a vital marker allowing the study of development of Phytophthora in vitr
o and in the host plant. (C) 1999 Federation of European Microbiological So
cieties. Published by Elsevier Science B.V. All rights reserved.