Degradation of Candida albicans Can1 permease expressed in Saccharomyces cerevisiae

The Candida albicans amino-acid Can1 permease expressed in Saccharomyces ce revisiae is degraded in the vacuole after internalisation by endocytosis. T he CaCan1 inactivation and degradation is slow and not inducible by ammoniu m ions or 'stress' conditions. Using Saccharomyces cerevisiae mutants defec tive in ubiquitin-protein ligase and ubiquitin-protein hydrolase we have sh own that the degradation of heterologous CaCan1 permease is ubiquitin depen dent. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.