Developmental anatomy and apical organization of the primary root of cork oak (Quercus suber L.)

Using histological techniques, we investigated the development and organiza tion of the primary root and the root apex of Quercus suber L. (cork oak), a Mediterranean woody species growing in semiarid conditions. The presence of a quiescent center was also investigated. The histogenesis of the vascul ar system begins with the maturation of the protophloem elements. Primary p hloem and xylem strands form alternately, and maturation proceeds centripet ally, although xylem differentiation does not reach the center of the root, which is occupied by a pith. Most protoxylem elements were fully mature we ll before the endodermis entered State I with the formation of the Casparia n strip. A continuous vascular cambium forms before the initiation of the p hellogen in the outermost pericyclic layer. In the primary root the apex ha s an open organization with four groups of initials: vascular cylinder init ials, cortical initials, lateral root-cap initials, and columella initials. The quiescent center includes the vascular initials and some derivatives. These features contrast with the radicle in mature and germinating embryos that have a closed organization with three groups of initials and a wider q uiescent center.