Nucleotide sequences and further characterization of human papillomavirus DNA present in the CaSki, SiHa and HeLa cervical carcinoma cell lines

The complete nucleotide sequences of the human papillomavirus type 16 (HPV- 16) variants present in the CaSki and SiHa cervical carcinoma cell lines an d the primary subgenomic HPV-18 variant present in the HeLa cervical carcin oma cell line were determined using overlapping bulk PCR products as templa tes. PCR-based methods were also used to characterize five previously unrep orted CaSki HPV-16 genomic disruptions and the 5' cellular-viral junction c ommon to all HeLa HPV-18 subgenomic structures.