Nucleotide sequences and further characterization of human papillomavirus DNA present in the CaSki, SiHa and HeLa cervical carcinoma cell lines

Authors
Citation
Jd. Meissner, Nucleotide sequences and further characterization of human papillomavirus DNA present in the CaSki, SiHa and HeLa cervical carcinoma cell lines, J GEN VIROL, 80, 1999, pp. 1725-1733
Citations number
60
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF GENERAL VIROLOGY
ISSN journal
00221317 → ACNP
Volume
80
Year of publication
1999
Part
7
Pages
1725 - 1733
Database
ISI
SICI code
0022-1317(199907)80:<1725:NSAFCO>2.0.ZU;2-X
Abstract
The complete nucleotide sequences of the human papillomavirus type 16 (HPV- 16) variants present in the CaSki and SiHa cervical carcinoma cell lines an d the primary subgenomic HPV-18 variant present in the HeLa cervical carcin oma cell line were determined using overlapping bulk PCR products as templa tes. PCR-based methods were also used to characterize five previously unrep orted CaSki HPV-16 genomic disruptions and the 5' cellular-viral junction c ommon to all HeLa HPV-18 subgenomic structures.