Jr. Schnell et al., Binding-induced activation of DNA alkylation by duocarmycin SA: Insights from the structure of an indole derivative-DNA adduct, J AM CHEM S, 121(24), 1999, pp. 5645-5652
The mechanism for catalysis of DNA alkylation by the potent antitumor antib
iotic duocarmycin SA (DSA) has been probed by determining the structure of
a DNA adduct of the indole analogue (DSA-indole, DSI) lacking three methoxy
functional groups. The three-dimensional structure of DSI covalently bound
to A(19) in d-(G(1)AC<(TAATT)under bar>GAC(11)).d-(G(12)TC<(AATTA)under ba
r>GTC(22)) was determined by H-1 NMR spectroscopy using a total of 935 expe
rimental distance and dihedral angle constraints. The representative ensemb
le of 20 conformers has no distance restraint violations greater than 0.03
Angstrom, no torsional restraint violations greater than 0.7 degrees, and a
pairwise rmsd over all atoms in the binding site of 0.48 Angstrom. compari
son of the structures of the DSA and DSI adducts reveals a structural basis
for the critical role of one of the trimethoxy-indole functional groups in
alkylation reactivity. A deeper penetration into the DNA minor groove in t
he vicinity of the indole subunit is observed for the DSI versus the DSA ad
duct, along with some variations in the width and depth of the minor groove
throughout the binding site. The most significant difference between the D
SI and DSA addducts is the 8 degrees smaller twist of the two ligand subuni
ts in DSI, which correlates with its similar to 20-fold slower rate of DNA
alkylation, This comparison of the structures of the DSI and DSA adducts to
the same DNA duplex provides the most direct evidence to date in support o
f the proposal that the binding of the ligand in the DNA minor groove and c
onsequent twisting of the two ligand subunits, disrupting vinylogous amide
stabilization and thereby activating the conjugated cyclopropane electrophi
le, plays a central role in controlling DNA alkylation reactivity.