The quantitative determination of rutin in different glycerinic plant extracts by solid-phase extraction and thin-layer chromatography with densitometry

Authors
Cobzac, S Cimpan, G Olah, N Gocan, S
Citation
S. Cobzac et al., The quantitative determination of rutin in different glycerinic plant extracts by solid-phase extraction and thin-layer chromatography with densitometry, J PL CHROM, 12(1), 1999, pp. 26-29
Citations number
16
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC
ISSN journal
09334173 → ACNP
Volume
12
Issue
1
Year of publication
1999
Pages
26 - 29
Database
ISI
SICI code
0933-4173(199901/02)12:1<26:TQDORI>2.0.ZU;2-V
Abstract
The quantitative determination of rutin from Ribes nigrum, Sorbus domestica , and Tilia tomentosa glycerinic plant extracts has been performed by TLC-d ensitometry, Solid-phase extraction (SPE) was used to separate the glycerin e from the plant extracts studied. The SPE recovery of rutin was 100.4%, i. e. loss of the substance of interest was negligible. Silica gel 60F(254) pl ates were scanned at lambda = 260 nm (UV, fluorescence quenching), and at l ambda = 440 nm (visible region) after spraying with 2-aminoethyldiphenylbor inate. Similar results obtained from the use of these two methods of detect ion.