Optochemical nanosensors and subcellular applications in living cells

What may be the smallest anthropogenic devices to date, spherical sensors ( wireless and fiber-less) with radii as small as 10 nm have been produced. T his class of optochemical PEBBLE (Probe Encapsulated By Biologically Locali zed Embedding) sensors covers a wide range of analytes (pH, calcium, oxygen and potassium included here) with excellent spatial, temporal and chemical resolution. Examples of such sensors for the monitoring of intracellular a nalytes are given. Methods, such as pico-injection, liposomal delivery and gene gun bombardment, are used to inject PEBBLE sensors into single cells. These PEBBLEs have caused minimal perturbation when delivered and operated inside single mammalian cells, such as human neuroblastoma, mouse oocytes o r rat alveolar macrophage.