Effects of imidacloprid on adult and larval stages of the flea Ctenocephalides felis after in vivo and in vitro application: a light- and electron-microscopy study

The effects of imidacloprid (Advantage(R)) on the larval and adult stages o f cat fleas (Ctenocephalides felis) were studied in vivo and in vitro by me ans of light and electron microscopy. It was found that: 1. The compound acted rapidly on both larval and adult fleas, killing both stages within 20 min of contact. 2. When applied as a spot-on to the skin of dogs, the compound localized in the water-resistant lipid layer of the skin surface and in the hairs but n ot in the blood. 3. Thus, the compound was not taken up during sucking of the flea but was a bsorbed via the thin intersegmental membranes, since larval and adult fleas that had only external contact with imidacloprid-impregnated paper or with shaved hairs from imidacloprid-treated dogs showed reactions similar to th ose shown by fleas sitting on treated skin. 4. The compound led to a continuous blockage of insect-specific nicotinic-a cetylcholine receptors (nAChR), causing tetanic muscle contractions within minutes of exposure. This manifested as intense trembling of the legs and p umping movements of the body. The affected flea stages remained motionless while the nerves and muscles were constantly and irreversibly destroyed due to hyperactivity. The ganglia of the head and thorax and the striated musc les of the flea body and legs were damaged first, whereas the intestinal mo vements (e.g., visible in larvae) took longer to exhibit damage. In summary, these studies show that imidacloprid kills larval and adult fle a stages rapidly via the same mode of action and thus prevents the developm ent of flea populations in human or animal dwellings.