Steroidal alkaloids and stilbenoids from Veratrum taliense

Phytochemical investigation of roots and rhizomes of Veratrum taliense yiel ded a new and six known steroidal alkaloids as well as a new and one report ed stilbene derivative. By a combination of spectral methods (IR, MS, H-1- and C-13-NMR, COSY, HMQC, HMBC, and NOESY), the structure of the new alkalo id was established as 15-angeloylgermine while the known ones were identifi ed as 15-(2-methylbutyroyl)germine, jervine, 3-veratroylzygadenine, germine , veramiline 3-O-beta-D-glucopyranoside and stenophylline B-3-O-beta-D-gluc opyranoside. The new stilbenoid, named veraphenol, was determined to be 2-( 3',5'-dihydroxyphenyl)-6-hydroxybenzofuran, and the known one was shown to be resveratrol. The in vitro enzyme assay indicated that 3-veratroylzygaden ine and resveratrol are inhibitors of xanthine oxidase. The enzyme inhibito ry action of resveratrol, the most active compound found so far in V. talie nse, is dose-dependent with the IC50 value at 30 mu M (the IC50 value of al lopurinol used as a positive control in the study is 10 mu M).