Heat capacity change for ribonuclease A folding

Authors
Pace, CN Grimsley, GR Thomas, ST Makhatadze, GI
Citation
Cn. Pace et al., Heat capacity change for ribonuclease A folding, PROTEIN SCI, 8(7), 1999, pp. 1500-1504
Citations number
46
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PROTEIN SCIENCE
ISSN journal
09618368 → ACNP
Volume
8
Issue
7
Year of publication
1999
Pages
1500 - 1504
Database
ISI
SICI code
0961-8368(199907)8:7<1500:HCCFRA>2.0.ZU;2-0
Abstract
The change in heat capacity Delta C-p for the folding of ribonuclease A was determined using differential scanning calorimetry and thermal denaturatio n curves. The methods gave equivalent results, Delta C-p = 1.15 +/- 0.08 kc al mol(-1) K-1 Estimates of the conformational stability of ribonuclease A based on these results from thermal unfolding are in good agreement with es timates from urea unfolding analyzed using the linear extrapolation method.