S-RNases in apple are expressed in the pistil along the pollen tube growthpath

Citation
Ac. Certal et al., S-RNases in apple are expressed in the pistil along the pollen tube growthpath, SEX PLANT R, 12(2), 1999, pp. 94-98
Citations number
29
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
SEXUAL PLANT REPRODUCTION
ISSN journal
09340882 → ACNP
Volume
12
Issue
2
Year of publication
1999
Pages
94 - 98
Database
ISI
SICI code
0934-0882(199906)12:2<94:SIAAEI>2.0.ZU;2-B
Abstract
The molecular bases of self-incompatibility have been intensively studied i n a restricted number of model species, but for most families the expressio n and distribution of S-proteins is unknown. In this work, pistil cryosecti ons from apple were used for in situ detection of S-proteins. Two specific antibodies, one against the S3-protein and another against all apple S-prot eins were used. S-proteins were shown to be localised in the intercellular space of the transmitting tissue, both in the stigma and style, which agree s with the proposed mechanism of action for S-RNases in gametophytic self-i ncompatibility. Some intracellular labelling was also observed in all ovary sections, confined to one layer of the nucellus surrounding the embryo sac , but this labelling was found to be non-S-allele-specific. Nevertheless, t he signal in the ovary was tissue-specific, which may indicate that some co mponent not encoded by the S-locus but similar to S-proteins was detected. To the best of our knowledge this is the first report on the precise distri bution of S-RNases in a rosaceous species.