PAC and cosmid contig spanning the HOXA cluster on human chromosome 7p15

To construct the PAC and cosmid contig map spanning the HOXA cluster on hum an chromosome 7, we used 9 DNA markers (D7S2243, D7S3010, HOXA1, EVX1, 750, pBH8, p60, p8.0, and HOXA11), among which the final 4 were generated in th is study by shotgun cloning strategy. From the libraries, 5 PAC and 35 cosm id clones were screened and as a result, an overlapping continuous array of cosmid and PAC clones covering the genomic region (about 200 kb) spanning the entire cluster were constructed. The isolated cosmids contained several consecutive HOX genes of regional group, probably sharing the regulatory p rocesses such as alternative splicing or polyadenylation, and thus could be used as useful materials for elucidating the molecular mechanism of HOX ge ne expression in the future.