We used the Matlock Periphytometer (Matlock et al., 1998) to measure in sit
u nutrient limitations and trophic status at five stream sires in the Bosqu
e River Watershed in north-central Texas during July 1997. Periphytic chlor
ophyll a production from the Matlock Periphytometer was also used as an ind
icator of baseline primary productivity and of maximum primary productivity
(MPP) in response to nutrient enrichment (nitrogen and phosphorus). The me
asured MPP was interpreted to represent the rate of periphytic growth when
nutrients are plot limiting. We developed a lotic ecosystem trophic status
index (LETSI) using the ratio of baseline primary productivity to MPP. Nitr
ogen (N) and phosphorus (P) limitations were evaluated using a modified LET
SI as the ratio of either N or P enriched growth to MPP. The LETSI is by de
finition a functional index, and may provide a classification tool for loti
c ecosystem trophic status. Using the LETSI indices, we observed difference
s in nutrient limitations in the streams and detected co-limitations of nit
rogen and phosphorus at two of the stream sites.