A method for measurement of subdermal fat thickness in dairy cows by digiti
zing cross-sections of ultrasonic scans was investigated. A commercial ultr
asonic system was modified for acquiring and presenting A-mode and B-mode u
ltrasonic images. Two sires were selected for a series of successive measur
ements: one was on the flat area of the rear of the rump between the pin bo
ne and the tail head; the other was between 12th and 13th ribs, below the b
ack rump. Several points were measured in each site. Two probe holders were
fabricated to fit each site and to ensure successive measurements at exact
ly the same points. A 7.5-MHz probe was used. Both A and B scans were condu
cted and recorded on videotape. A computer program was written to acquire a
nd digitize data from A-mode scans to a depth of 30 mm. An algorithm locate
d signal peaks to distinguish among skin, subdermal fat and muscle, and the
n calculated thickness of layers at each point. A trial was conducted to ev
aluate the use of this system to detect body condition changes of the dairy
cow during lactation. Six cows were monitored and measured from calving to
late lactation: subdermal fat was ultrasonically measured weekly, and cows
were scared weekly for body condition and weighed daily. It was concluded
that successful ultrasonic measurements for body condition assessment, to d
etect energy balance changes, depend on accurate and reliable standardizati
on of measurements, at a responsive site. The between-ribs site was more re
sponsive, and a single point (usually just below the spine) provided suffic
ient data for the required purpose. Given the conditions described, the sys
tem can measure quantitatively changes of subdermal fat thickness in lactat
ing dairy cows.