HLA CLASS I-RESTRICTED AND TUMOR-SPECIFIC CYTOTOXIC T-LYMPHOCYTES FROM METASTATIC LYMPH-NODES OF ESOPHAGEAL CANCERS

This paper investigates the presence of HLA class I-restricted and tum or-specific cytotoxic T lymphocytes (CTL) in tumor sites of esophageal cancers. Five CTL lines were established from the metastatic lymph no des or pleural effusion by incubation with interleukin-2 of tumor-infi ltrating lymphocytes: cases 1 and 5, HLA-A26- and HLA-A33-restricted a nd squamous cell carcinoma (SCC)-specific CTL; case 2, HLA-Cw0102-rest ricted and esophageal SCC-specific CTL; case 3, HLA-A24- and HLA-A26-r estricted CTL recognizing histologically different tumor cells; and ca se 4, HLA-A26-restricted and esophageal SCC-specific CTL. These result s suggest the existence of HLA class I-restricted and tumor-specific C TL in metastatic esophageal SCC. (C) 1997 Academic Press.