Leginon: a system for fully automated acquisition of 1000 electron micrographs a day

Authors
Potter, CS Chu, H Frey, B Green, C Kisseberth, N Madden, TJ Miller, KL Nahrstedt, K Pulokas, J Reilein, A Tcheng, D Weber, D Carragher, B
Citation
Cs. Potter et al., Leginon: a system for fully automated acquisition of 1000 electron micrographs a day, ULTRAMICROS, 77(3-4), 1999, pp. 153-161
Citations number
15
Categorie Soggetti
Multidisciplinary,"Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ULTRAMICROSCOPY
ISSN journal
03043991 → ACNP
Volume
77
Issue
3-4
Year of publication
1999
Pages
153 - 161
Database
ISI
SICI code
0304-3991(199907)77:3-4<153:LASFFA>2.0.ZU;2-Q
Abstract
We have developed a system to automatically acquire large numbers of accept able quality images from specimens of negatively stained catalase, a biolog ical protein which forms crystals. In this paper we will describe the detai ls of the system architecture and analyze the performance of the system as compared to a human operator. The ultimate goal of the system if to automat e the process of acquiring cryo-electron micrographs, (C) 1999 Elsevier Sci ence B.V. All rights reserved.