Characterization of bovine coronavirus isolates from eight different states in the USA

Bovine coronavirus isolates from eight different states of the USA were com pared for their antigenic properties and susceptibility to hygromycin B. An tigenic differences were observed among the isolates in a one-way hemagglut ination-inhibition (HI) test using a polyclonal antiserum against the Mebus bovine coronavirus isolate. Differences were observed on isoelectric focus ing among viral proteins with isoelectric points between 4.45-4.65. Most of the BCV isolates were susceptible to hygromycin B (0.5 mM) whereas a few h ygromycin B resistant isolates were also found. (C) 1999 Elsevier Science B .V. All rights reserved.